MARCKSL1 Polyklonaler Antikörper

MARCKSL1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11422-1-AP

Synonyme

F52, Mac MARCKS, MACMARCKS, MARCKS like 1, MARCKS like protein 1, MARCKS related protein, MARCKSL1, MLP, MLP1, MRP

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Galerie der Validierungsdaten

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  WB analysis of human brain using 11422-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11422-1-AP (MARCKSL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11422-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11422-1-AP (MARCKSL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11422-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11422-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11422-1-AP bindet in WB, IHC, ELISA MARCKSL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARCKSL1 fusion protein Ag1967
• Vollständiger Name: MARCKS-like 1
• Berechnetes Molekulargewicht: 195 aa, 20 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC007904
• Gene symbol: MARCKSL1
• Gene ID (NCBI): 65108
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, it is also named MARCKS-like protein (MLP) or MARCKS-related protein (MRP) and belongs to the MARCKS family, which is a highly acidic myristoylated family of PKC substrates widely distributed in diverse cell types including macrophages. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation. Genetic disruption of MARCKSL1 results in neural tube closure defects, events that depend on coordinated control of actin functions, cell shape, and cell migration. MARCKSL1 are thought to regulate the actin cytoskeleton and thereby participate in major cellular responses such as phagocytosis, secretion, motility, mitogenesis, and membrane trafficking.

Publikationen

Species	Application	Title
mouse	WB	J Proteome Res; Sparc-like protein 1 is a new marker of human glioma progression.; Authors - Turtoi Andrei A