MAPKAPK2 Polyklonaler Antikörper

MAPKAPK2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 13949-1-AP

Synonyme

MAPKAP-K2, MAPKAPK 2, MAPKAP kinase 2, MAPK-activated protein kinase 2, MAP kinase-activated protein kinase 2

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Galerie der Validierungsdaten

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  WB analysis using 13949-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13949-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13949-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 13949-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13949-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13949-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); F-actin is stained using CL555-phalloidin (red).

• 

  IF Staining of HeLa using 13949-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Maus-Kolongewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 2 publications below

Produktinformation

13949-1-AP bindet in WB, IHC, IF/ICC, ELISA MAPKAPK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAPKAPK2 fusion protein Ag5060
• Vollständiger Name: mitogen-activated protein kinase-activated protein kinase 2
• Berechnetes Molekulargewicht: 400 aa, 46 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC036060
• Gene symbol: MAPKAPK2
• Gene ID (NCBI): 9261
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAPKAPK2(mitogen-activated protein kinase-activated protein kinase 2) is also named as MK2, MAPKAP-K2, MK-2 and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK2, one of several kinases directly phosphorylated and activated by p38 MAPK, plays a central role in the inflammatory response and is in the nucleus of unstimulated cells and moves rapidly to the cytoplasm after stimulation(PMID:12171911). It is also involved in many other cellular processes including stress responses, nuclear export, gene expression regulation and cell proliferation. Multiple residues of MAPKAPK2 are generally phosphorylated in vivo in response to stress, but only 4 residues(Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in vitro(PMID:22351694). It has 2 isoforms produced by alternative splicing and the range of the molecular weight is 42-60 kDa according to the references(PMID:10666409; 11328854;8995385).

Publikationen

Species	Application	Title
human	WB	Mol Cancer Res; Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2.; Authors - Rui Wang
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Toxicol Appl Pharmacol; p38 MAPK-DRP1 signaling is involved in mitochondrial dysfunction and cell death in mutant A53T α-synuclein model of Parkinson's disease.; Authors - Chen Gui
human	WB	Int J Mol Med; Aryl hydrocarbon receptor inhibits inflammation in DSS‑induced colitis via the MK2/p‑MK2/TTP pathway.; Authors - Qimeng Wang
mouse	WB	Brain Res Bull; The p38 mitogen activated protein kinase regulates β-amyloid protein internalization through the α7 nicotinic acetylcholine receptor in mouse brain.; Authors - Kai-Ge Ma
mouse	WB	Cell Rep; MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal.; Authors - Yan Zhang