TAB2 Polyklonaler Antikörper

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• KD/KO Validated

TAB2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 14410-1-AP

Synonyme

KIAA0733, MAP3K7IP2, TAB 2, TAB2, TAK1 binding protein 2

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Galerie der Validierungsdaten

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  WB analysis using 14410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14410-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 14410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14410-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14410-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14410-1-AP

  IP Result of anti-TAB2 (IP:14410-1-AP, 4ug; Detection:14410-1-AP 1:600) with HepG2 cells lysate 3600ug.

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  IHC staining of human malignant melanoma using 14410-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human malignant melanoma using 14410-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 14410-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAP3K7IP2 antibody 14410-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14410-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug TAB2 antibody (14410-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, C6-Zellen, HepG2-Zellen, humanes Lebergewebe, Mauslebergewebe, Raji-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

14410-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TAB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAB2 fusion protein Ag5742
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 7 interacting protein 2
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC035910
• Gene symbol: MAP3K7IP2
• Gene ID (NCBI): 23118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tab2 has a role in the inflammatory signal transduction pathway and is involved in the reversal of NCoR-dependent and NFkB- or APP-mediated gene repression and androgen antagonist response. Besides, it is identified as a cofactor for ERBB4 to assemble NCoR corepressor complex in response to neuregulin [PMID:16469706]. It also functions as an adaptor that links TAK1 and TRAF6 in response to interleukin-1β (IL-1β) or tumour necrosis factor-α (TNF-α), and thereby mediates TAK1 activation [PMID:11460167].

Publikationen

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Nat Commun; Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.; Authors - Chunjie Sheng
human	WB	Cell Biol Toxicol; microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro.; Authors - Qiuyue Li ; KD Validated
mouse	WB	Front Immunol; MicroRNA 27a-3p Regulates Antimicrobial Responses of Murine Macrophages Infected byMycobacterium aviumsubspeciesparatuberculosisby Targeting Interleukin-10 and TGF-β-Activated Protein Kinase 1 Binding Protein 2.; Authors - Tariq Hussain
human	WB	J Neuroinflammation; miR-155 and miR-146a collectively regulate meningitic Escherichia coli infection-mediated neuroinflammatory responses.; Authors - Bo Yang
human	WB	Front Immunol; Hepatitis B Surface Antigen Suppresses the Activation of Nuclear Factor Kappa B Pathway via Interaction With the TAK1-TAB2 Complex.; Authors - Feiyan Deng