MEK1/2 Polyklonaler Antikörper

MEK1/2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

56

Kat-Nr. : 11049-1-AP

Synonyme

MAP2K2, MEK2, MAPK/ERK kinase 2, MAP kinase kinase 2, ERK activator kinase 2

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Galerie der Validierungsdaten

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  WB analysis using 11049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11049-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 11049-1-AP

  IP result of anti-MEK1/2 (IP:11049-1-AP, 4ug; Detection:11049-1-AP 1:1000) with HL-60 cells lysate 1280ug.

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  IHC staining of mouse brain using 11049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 11049-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (11049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 11049-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11049-1-AP (MEK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Hirngewebe, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 55 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11049-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MEK1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEK1/2 fusion protein Ag1481
• Vollständiger Name: mitogen-activated protein kinase kinase 2
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44-47 kDa
• GenBank-Zugangsnummer: BC018645
• Gene symbol: MEK2
• Gene ID (NCBI): 5605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.

Publikationen

Species	Application	Title
human	WB	Exp Mol Med; Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer; Authors - Yanmei Peng
mouse	WB	Nat Commun; ZFP281-BRCA2 prevents R-loop accumulation during DNA replication.; Authors - Yan Wang
mouse	WB	ACS Appl Mater Interfaces; Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.; Authors - Chaoqun Li
mouse,human	WB	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB	J Med Chem; Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.; Authors - Zongyuan Zhou
human	WB	Cell Death Dis; The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.; Authors - Wenjuan Li