MAP1B Monoklonaler Antikörper

MAP1B Monoklonal Antikörper für FC, IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E6B9

Kat-Nr. : 67423-1-Ig

Synonyme

DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP 1B, MAP1B, MAP5

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Galerie der Validierungsdaten

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  IHC staining of rat brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 67423-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67423-1-Ig (MAP1B antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of SH-SY5Y using 67423-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human MAP1B (67423-1-Ig, Clone:1E6B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67423-1-Ig bindet in IHC, IF, FC, ELISA MAP1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MAP1B fusion protein Ag17709
• Vollständiger Name: microtubule-associated protein 1B
• Berechnetes Molekulargewicht: 2468 aa, 271 kDa
• GenBank-Zugangsnummer: BC141853
• Gene symbol: MAP1B
• Gene ID (NCBI): 4131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)