MALT1 Polyklonaler Antikörper

MALT1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11660-1-AP

Synonyme

MALT1, MLT, MLT1, Paracaspase

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11660-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11660-1-AP (MALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11660-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11660-1-AP (MALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11660-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11660-1-AP (MALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11660-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11660-1-AP (MALT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 11660-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11660-1-AP (MALT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11660-1-AP

  Immunofluorescent analysis of HepG2 cells, using MALT1 antibody 11660-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 11660-1-AP

  Immunofluorescent analysis of HepG2 cells using 11660-1-AP (MALT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11660-1-AP

  1X10^6 HeLa cells were stained with 0.2ug MALT1 antibody (11660-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11660-1-AP bindet in WB, IHC, IF, FC, CoIP, ELISA MALT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MALT1 fusion protein Ag2182
• Vollständiger Name: mucosa associated lymphoid tissue lymphoma translocation gene 1
• Berechnetes Molekulargewicht: 824 aa, 92 kDa
• Beobachtetes Molekulargewicht: 92-100 kDa
• GenBank-Zugangsnummer: BC030143
• Gene symbol: MALT1
• Gene ID (NCBI): 10892
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.

Publikationen

Species	Application	Title
rat	WB	Eur J Pharmacol; Inhibition of MALT1 reduces ferroptosis in rat hearts following ischemia/reperfusion via enhancing the Nrf2/SLC7A11 pathway; Authors - Ya-Qian Jiang
human,mouse	WB	ACS Chem Neurosci; Telaprevir Improves Memory and Cognition in Mice Suffering Ischemic Stroke via Targeting MALT1-Mediated Calcium Overload and Necroptosis; Authors - Yi-Yue Zhang
human	IHC	Biomedicines; Mucosa-Associated Lymphoid Tissue 1 Is an Oncogene Inducing Cell Proliferation, Invasion, and Tumor Growth via the Upregulation of NF-κB Activity in Human Prostate Carcinoma Cells.; Authors - Ke-Hung Tsui
rat	IHC	Food Funct; Allicin suppressed Escherichia coli-induced urinary tract infections by a novel MALT1/NF-κB pathway.; Authors - Zhenglin Chang
mouse,rat	WB	Arch Biochem Biophys; MALT1 promotes necroptosis in stroke rat brain via targeting the A20/RIPK3 pathway; Authors - Zi-Mei Peng
rat	WB,IF,CoIP	Mol Neurobiol; The Weakened Interaction Between HECTD4 and GluN2B in Ischemic Stroke Promotes Calcium Overload and Brain Injury Through a Mechanism Involving the Decrease of GluN2B and MALT1 Ubiquitination; Authors - Yi-Yue Zhang ; KD Validated