MAGEC2 Polyklonaler Antikörper

MAGEC2 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10280-1-AP

Synonyme

Cancer/testis antigen 10, CT10, HCA587, MAGE C2 antigen, MAGE E1 antigen, MAGEC2, MAGEE1, melanoma antigen family C, 2, Melanoma associated antigen C2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10280-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10280-1-AP (MAGEC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human malignant melanoma using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10280-1-AP (MAGEC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10280-1-AP (MAGEC2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hodengewebe, humanes malignes Melanomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

10280-1-AP bindet in IHC, ELISA MAGEC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAGEC2 fusion protein Ag0418
• Vollständiger Name: melanoma antigen family C, 2
• Berechnetes Molekulargewicht: 373 aa, 41 kDa
• GenBank-Zugangsnummer: BC005891
• Gene symbol: MAGEC2
• Gene ID (NCBI): 51438
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Melanoma antigen family C, 2(MAGEC2, synonyms: CT10, HCA587, MAGEE1, MGC13377) is related to members of the MAGEC gene family. MAGEC2 is not expressed in normal tissues, except for testis, and is expressed in tumors of various histological types. This gene and other MAGEC genes are clustered on chromosome Xq26-q27.

Publikationen

Species	Application	Title
human	IHC	PLoS One; γ-Radiation promotes immunological recognition of cancer cells through increased expression of cancer-testis antigens in vitro and in vivo.; Authors - Sharma Anu A
human	IHC	Hum Pathol; Primary mediastinal seminomas: a comprehensive immunohistochemical study with a focus on novel markers.; Authors - Annikka Weissferdt
		Laryngoscope Investig Otolaryngol; The expression of the cancer testis antigen MAGE A4: A favorable prognostic biomarker in salivary gland carcinomas related to low tumor grading.; Authors - Domenic Vital