MAFF Polyklonaler Antikörper

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• KD/KO Validated

MAFF Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 12771-1-AP

Synonyme

hMafF, MAFF, Transcription factor MafF, U MAF

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Galerie der Validierungsdaten

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  WB analysis of human lung using 12771-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12771-1-AP (MAFF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SKOV-3 using 12771-1-AP

  IP result of anti-MAFF (IP:12771-1-AP, 4ug; Detection:12771-1-AP 1:500) with SKOV-3 cells lysate 3200 ug.

• 

  IHC staining of human ovary tumor using 12771-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12771-1-AP (MAFF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 12771-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12771-1-AP (MAFF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12771-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12771-1-AP (MAFF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lungengewebe
• Erfolgreiche IP: SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

12771-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA MAFF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAFF fusion protein Ag3460
• Vollständiger Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
• Berechnetes Molekulargewicht: 17 aa, 8 kDa
• Beobachtetes Molekulargewicht: 18-23 kDa
• GenBank-Zugangsnummer: BC015037
• Gene symbol: MAFF
• Gene ID (NCBI): 23764
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

Publikationen

Species	Application	Title
human	WB,IHC,IF	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
human,mouse	WB,IHC,IF	J Clin Invest; circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.; Authors - Zhenzhen Chen
human	WB	Autophagy; The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3.; Authors - Eutteum Jeong
mouse,rat	WB	Mol Ther Nucleic Acids; miR-320a induces pancreatic β cells dysfunction in diabetes by inhibiting MafF; Authors - Hengzhi Du
human	WB	Mol Ther Oncolytics; RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.; Authors - Shuyuan Shen ; KD Validated
mouse	WB	Dis Markers; Responsive Expression of MafF to β-Amyloid-Induced Oxidative Stress.; Authors - Xiaoxuan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amy (Verified Customer) (03-18-2024)

Relatively clean bands but not very strong, U2OS lysates 25ug.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: U2OS