MAB21L2 Polyklonaler Antikörper

MAB21L2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 10971-1-AP

Synonyme

Protein mab-21-like 2, Protein mab 21 like 2, mab 21 like 2 (C. elegans)

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10971-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10971-1-AP (MAB21L2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of Human Colon cancer using 10971-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 10971-1-AP (MAB21L2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10971-1-AP bindet in WB, IHC, ELISA MAB21L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAB21L2 fusion protein Ag1423
• Vollständiger Name: mab-21-like 2 (C. elegans)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC009983
• Gene symbol: MAB21L2
• Gene ID (NCBI): 10586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mab-21 gene has been identified as a critical component required for sensory organ identity establishment in Caenorhabditis elegans. Mab21l1 and Mab21l2 paralogs have widespread and dynamic expression patterns during vertebrate development. Depletion of Mab21l1 and Mab21l2 messages in mouse embryo arrests axial turning, and impairs notochord and neural tube differentiation. Human MAB21L2 gene encodes mab-21-like 2 protein belonging to the mab-21 family. The Mab21l2-deficient mice have defects in eye and body wall formation, indicating that Mab21l2 plays crucial roles in retina and in ventral body wall formation. MAB21L2 plays a crucial role in both heart and liver development during mouse embryogenesis. This antibody is supposed to recognize both MAB21L2 and its homolog MAB21L1.

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); STAMP2 Expression Mediated by Cytokines Attenuates Their Growth-Limiting Effects in Prostate Cancer Cells.; Authors - Nicklas Pihlstrøm

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kathryn (Verified Customer) (04-07-2023)

No band appeared where expected. The antibody produced a band in the protein ladder at approximately 66 kDa, possibly BSA. Ultimately, this antibody does not detect the protein of interest

• Applications: Western Blot
• Primary Antibody Dilution: 1:200, 1:100 tried
• Cell Tissue Type: E12.5 mouse head expressing epitope tagged Mab21l2

MAB21L2 Antibody Western Blot validation (1:200, 1:100 tried dilution) in E12.5 mouse head expressing epitope tagged Mab21l2 (Cat no:10971-1-AP)