Lamin B1 Monoklonaler Antikörper

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• KD/KO Validated

Lamin B1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

3C10G12

Kat-Nr. : CL555-66095

Synonyme

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

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Galerie der Validierungsdaten

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  IF Staining of MCF-7 using CL555-66095

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®555 Lamin B1 antibody (CL555-66095, Clone: 3C10G12 ) at dilution of 1:800.

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  IF Staining of U2OS using CL555-66095

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite®555 Lamin B1 antibody (CL555-66095, Clone: 3C10G12 ) at dilution of 1:1000.

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  FC experiment of HeLa using CL555-66095

  1X10^6 HeLa cells were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human Lamin B1 (CL555-66095, Clone:3C10G12) (red), or 0.25 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: MCF-7-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66095 bindet in IF, FC (Intra) Lamin B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lamin B1 fusion protein Ag20522
• Vollständiger Name: lamin B1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC012295
• Gene symbol: LMNB1
• Gene ID (NCBI): 4001
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.