LY96/MD2 Polyklonaler Antikörper

LY96/MD2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11784-1-AP

Synonyme

ESOP 1, ESOP1, Ly 96, LY96, LY96/MD2, lymphocyte antigen 96, MD 2, MD2, Protein MD 2

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Galerie der Validierungsdaten

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  WB analysis using 11784-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11784-1-AP (LY96/MD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 11784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11784-1-AP (LY96/MD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11784-1-AP (LY96/MD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11784-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA LY96/MD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LY96/MD2 fusion protein Ag2447
• Vollständiger Name: lymphocyte antigen 96
• Berechnetes Molekulargewicht: 160 aa, 18 kDa
• Beobachtetes Molekulargewicht: 20-26 kDa
• GenBank-Zugangsnummer: BC020690
• Gene symbol: MD2
• Gene ID (NCBI): 23643
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LY96, also named as ESOP1 or MD 2, is a small secreted glycoprotein that can bind to both the hydrophobic portion of LPS and to the extracellular domain of TLR4. The interaction between MD-2 and LPS bridges the two TLR4 molecules and induces the dimerization of LPS-MD-2-TLR4, which forms the structural basis for biological functions of TLR4/MD-2 complex. Due to its essential role in mediating the interaction between LPS and TLR4, MD-2 has been extensively explored as a therapeutic target for treatment of inflammatory disorders.

Publikationen

Species	Application	Title
human	WB	Front Oncol; MD2 Is a Potential Biomarker Associated with Immune Cell Infiltration in Gliomas.; Authors - Mengya Zhao
mouse	WB	J Cardiovasc Transl Res; Macrophage CD36 and TLR4 Cooperation Promotes Foam Cell Formation and VSMC Migration and Proliferation Under Circadian Oscillations.; Authors - Zhen Sun
rat		Molecules; Sal B Alleviates Myocardial Ischemic Injury by Inhibiting TLR4 and the Priming Phase of NLRP3 Inflammasome.; Authors - Yang Hu
human	WB	Oncol Rep; Curcumin inhibits cell proliferation and migration in NSCLC through a synergistic effect on the TLR4/MyD88 and EGFR pathways.; Authors - Lanfeng Zhang
mouse	WB,IF,IP	J Agric Food Chem; Biotransformation of 18β-Glycyrrhetinic Acid by Human Intestinal Fungus Aspergillus niger RG13B1 and the Potential Anti-Inflammatory Mechanism of Its Metabolites; Authors - Min Zhang
mouse	WB,IF	Bioorg Chem; The antimicrobial peptide LK2(6)A(L) exhibits anti-inflammatory activity by binding to the myeloid differentiation 2 domain and protects against LPS-induced acute lung injury in mice; Authors - Juan Zhang