LTF Polyklonaler Antikörper

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• KD/KO Validated

LTF Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10933-1-AP

Synonyme

GIG12, HLF2, Lactoferrin, lactotransferrin, LF, LTF, Talalactoferrin

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Galerie der Validierungsdaten

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  WB analysis using 10933-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10933-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human pancreas using 10933-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10933-1-AP

  IP result of anti-LTF (IP:10933-1-AP, 4ug; Detection:10933-1-AP 1:600) with HeLa cells lysate 4000ug.

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  IHC staining of human liver using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human prostate hyperplasia using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human saliva, HEK-293-Zellen, humanes Pankreasgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

10933-1-AP bindet in WB, IP, IHC, ELISA LTF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LTF fusion protein Ag1379
• Vollständiger Name: lactotransferrin
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 30,51,80 kDa
• GenBank-Zugangsnummer: BC015822
• Gene symbol: LTF
• Gene ID (NCBI): 4057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).

Publikationen

Species	Application	Title
human	IHC	Sci Adv; Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.; Authors - Takafumi Kato
human	WB,IHC	Cancer Cell Int; Identification and validation of an immune-related prognostic signature and key gene in papillary thyroid carcinoma.; Authors - Rujia Qin
human	WB	Biochem Biophys Res Commun; NEDD4L-mediated LTF protein degradation limits ferroptosis.; Authors - Yuan Wang ; KD Validated
human	IHC	Int Med Case Rep J; Collagen fiber changes related to keratoconus with secondary corneal amyloidosis.; Authors - Kaoru Araki-Sasaki
mouse	WB	Bioact Mater; Passively-targeted mitochondrial tungsten-based nanodots for efficient acute kidney injury treatment; Authors - Qiong Huang