LSM8 Polyklonaler Antikörper

LSM8 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10134-1-AP

Synonyme

YJR022W, U6 snRNA-associated Sm-like protein LSm8, NAA38

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10134-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10134-1-AP (LSM8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10134-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10134-1-AP (LSM8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 10134-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10134-1-AP (LSM8 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 10134-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10134-1-AP (LSM8 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 10134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LSM8 antibody (10134-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10134-1-AP bindet in WB, IHC, IF/ICC, ELISA LSM8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LSM8 fusion protein Ag0178
• Vollständiger Name: LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• Berechnetes Molekulargewicht: 14 kDa /75 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC002742
• Gene symbol: LSM8
• Gene ID (NCBI): 51691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing[PMID:15075370]. Lsm2-Lsm8 (Lsm stands for Like Sm), binds and stabilizes the 3′ end of the spliceosomal U6 snRNA Lsm proteins facilitate the annealing of U6 snRNA with U4 snRNA in vitro and interact with the U4/U6 annealing factor Prp24, suggesting that Lsm proteins function in U4/U6 snRNP formation in vivo [PMID:15485930, 17251193].

Publikationen

Species	Application	Title
human	WB	Mol Cell; LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.; Authors - Xin Wang