LSM4 Polyklonaler Antikörper

LSM4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10834-1-AP

Synonyme

YER112W, U6 snRNA-associated Sm-like protein LSm4, GRP, Glycine-rich protein, Glycine rich protein

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 10834-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10834-1-AP (LSM4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10834-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10834-1-AP (LSM4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-231 using 10834-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 10834-1-AP (LSM4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10834-1-AP (LSM4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10834-1-AP (LSM4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10834-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10834-1-AP (LSM4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, humanes Plazenta-Gewebe, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10834-1-AP bindet in WB, IHC, IF/ICC, ELISA LSM4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LSM4 fusion protein Ag1280
• Vollständiger Name: LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC022198
• Gene symbol: LSM4
• Gene ID (NCBI): 25804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sm-like proteins, such as LSM4, were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing

Publikationen

Species	Application	Title
human	WB	EMBO Rep; EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics.; Authors - Jeremiah J Bearss
human	WB	Mol Cell Biol; A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.; Authors - Berg Michael G MG