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LSM1 Polyklonaler Antikörper

LSM1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10259-1-AP

Synonyme

Cancer associated Sm like, CASM, LSM1, Small nuclear ribonuclear CaSm, YJL124C

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HeLa cells using LSM1 Polyclonal antibody (10259-1-AP)

    WB analysis of HeLa using 10259-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10259-1-AP (LSM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using LSM1 Polyclonal antibody (10259-1-AP)

    WB analysis of mouse kidney using 10259-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10259-1-AP (LSM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LSM1 Polyclonal antibody (10259-1-AP)

    IP experiment of HeLa using 10259-1-AP

    IP result of anti-LSM1 (IP:10259-1-AP, 4ug; Detection:10259-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LSM1 Polyclonal antibody (10259-1-AP)

    IHC staining of human prostate cancer using 10259-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10259-1-AP (LSM1 antibody) at dilution of 1:100 (under 10x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, Mausnierengewebe
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10259-1-AP bindet in WB, IP, IHC, ELISA LSM1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LSM1 fusion protein Ag0232
Vollständiger Name LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Berechnetes Molekulargewicht 15 kDa
Beobachtetes Molekulargewicht 18-20 kDa
GenBank-ZugangsnummerBC001767
Gene symbol LSM1
Gene ID (NCBI) 27257
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lsm1 is a component of the Lsm1-7 complex involved in cytoplasmic mRNA degradation. Lsm1 is a key subunit that determines the RNA-binding properties of this complex. The normal RNA-binding activity of this complex is crucial for mRNA decay and 3'-end protection in vivo and requires the intact Sm-domain of Lsm1. LSM1 localizes to cytoplasmic processing bodies (P bodies) involved in mRNA storage, processing, regulation, and degradation and is required for P-body formation. LSM1 proteins facilitate the formation of U4/U6 RNA duplexes in vitro and may play a role in U4/U6 snRNP formation.