LRRC8D Polyklonaler Antikörper

LRRC8D Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11537-1-AP

Synonyme

Volume-regulated anion channel subunit LRRC8D, LRRC5, Leucine-rich repeat-containing protein 8D, Leucine-rich repeat-containing protein 5, HsLRRC8D

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11537-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11537-1-AP (LRRC8D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11537-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11537-1-AP (LRRC8D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 11537-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11537-1-AP (LRRC8D antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 11537-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11537-1-AP (LRRC8D antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11537-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11537-1-AP (LRRC8D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11537-1-AP bindet in WB, IHC, IF/ICC, ELISA LRRC8D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRRC8D fusion protein Ag2138
• Vollständiger Name: leucine rich repeat containing 8 family, member D
• Berechnetes Molekulargewicht: 858 aa, 98 kDa
• Beobachtetes Molekulargewicht: 98-120 kDa
• GenBank-Zugangsnummer: BC024159
• Gene symbol: LRRC8D
• Gene ID (NCBI): 55144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A.

Publikationen

Species	Application	Title
human	IHC	Cell Physiol Biochem; Cell Volume Regulation in the Epidermis.; Authors - Magdalena Jahn
human	WB	Cell Genom; Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders; Authors - Greta Pintacuda