PTPRA Polyklonaler Antikörper

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• KD/KO Validated

PTPRA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13079-1-AP

Synonyme

LRP, HPTPalpha, HPTPA, HLPR, HEPTP

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Galerie der Validierungsdaten

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  WB analysis of rat liver using 13079-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13079-1-AP (PTPRA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13079-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13079-1-AP (PTPRA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 13079-1-AP

  IP result of anti-PTPRA (IP:13079-1-AP, 4ug; Detection:13079-1-AP 1:300) with A431 cells lysate 2400ug.

• 

  IHC staining of rat heart using 13079-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 13079-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13079-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 13079-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13079-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13079-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 13079-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PTPRA antibody (13079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, MCF-7-Zellen, Rattenlebergewebe, SKOV-3-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Urothelkarzinomgewebe, Mausherzgewebe, Mausnierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

13079-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PTPRA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPRA fusion protein Ag3760
• Vollständiger Name: protein tyrosine phosphatase, receptor type, A
• Berechnetes Molekulargewicht: 793 aa, 91 kDa
• Beobachtetes Molekulargewicht: 90-105 kDa, 140 kDa
• GenBank-Zugangsnummer: BC027308
• Gene symbol: PTPRA
• Gene ID (NCBI): 5786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncol Lett; PTPRA facilitates cancer growth and migration via the TNF-α-mediated PTPRA-NF-κB pathway in MCF-7 breast cancer cells.; Authors - Canfeng Lin ; KO Validated
human	WB,IF,IP	Oncogene; Glucose-mediated N-glycosylation of RPTPα affects its subcellular localization and Src activation; Authors - Jiayu Fang
mouse	WB	Sci Signal; Clustering of phosphatase RPTPα promotes Src signaling and the arthritogenic action of synovial fibroblasts; Authors - Sho Sendo
human	WB	EBioMedicine; The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.; Authors - Siliang Wei ; KD Validated