LRDD Polyklonaler Antikörper

LRDD Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12119-1-AP

Synonyme

PIDD-C, PIDD1, PIDD, p53-induced death domain-containing protein 1, Leucine-rich repeat and death domain-containing protein

---

Galerie der Validierungsdaten

• 

  WB analysis of L02 using 12119-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12119-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12119-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 12119-1-AP

  IP result of anti-LRDD (IP:12119-1-AP, 4ug; Detection:12119-1-AP 1:500) with L02 cells lysate 2800ug.

• 

  IHC staining of human pancreas using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 12119-1-AP

  Immunofluorescent analysis of Hela cells, using LRDD antibody 12119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HEK-293 using 12119-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRDD antibody (12119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HEK-293-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12119-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LRDD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRDD fusion protein Ag2761
• Vollständiger Name: leucine-rich repeats and death domain containing
• Berechnetes Molekulargewicht: 893 aa, 98 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC014904
• Gene symbol: LRDD
• Gene ID (NCBI): 55367
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P53-induced protein with a death domain (PIDD/LRDD) is a component of the PIDDosome. PIDD contains 910 residues with 7 leucine rich repeats (LRRs), 2 ZU-5 domains and a C-terminal death domain (DD). PIDD can be cleaved into shorter fragments generating a PIDD-N fragment of 48 kD (residues 1-446), a PIDD-C fragment of 51 kD (residues 447-910) and a PIDD-CC fragment of 37 kD (residues 589-910). Auto-cleavage of PIDD determines the downstream signaling events. The PIDD-C fragment mediates activation of NFκB via the recruitment of RIP1 and NEMO, while PIDD-CC causes caspase-2 activation, which leads to apoptosis.

Publikationen

Species	Application	Title
mouse	WB	Neural Regen Res; Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation.; Authors - Wei-Na Chai
mouse	WB	Exp Neurol; Microglia aggravate white matter injury via C3/C3aR pathway after experimental subarachnoid hemorrhage; Authors - Lei Yang