LRAT Polyklonaler Antikörper

LRAT Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12815-1-AP

Synonyme

LRAT



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushodengewebe, Y79-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 3 publications below

Produktinformation

12815-1-AP bindet in WB, ELISA LRAT und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte ReaktivitätMaus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LRAT fusion protein Ag3556
Vollständiger Name lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
Berechnetes Molekulargewicht 230 aa, 26 kDa
Beobachtetes Molekulargewicht 26 kDa, 50-54 kDa
GenBank-ZugangsnummerBC031053
Gene symbol LRAT
Gene ID (NCBI) 9227
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.

Protokolle

Produktspezifische Protokolle
WB protocol for LRAT antibody 12815-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratWB

iScience

Control by the brain of vitamin A homeostasis

Authors - Peter I Imoesi
ratWB

Am J Pathol

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.

Authors - Volha V Malechka
mouseWB

J Food Biochem

Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.

Authors - Wei Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Peter (Verified Customer) (12-03-2018)

The Proteintech LRAT primary antibody works so well and the antibody is prepared using BSA it can be use repeatedly when stored at -20. The strength of the antibody remains the same over a period of 6 months or more.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rat Liver Tissue
LRAT Antibody Western Blot, validation (1:500 dilution) in Rat Liver Tissue (Cat no:12815-1-AP)