LONP1 Monoklonaler Antikörper

LONP1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1C6C12

Kat-Nr. : CL594-66043

Synonyme

hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15

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Galerie der Validierungsdaten

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  IF Staining of C6 using CL594-66043

  Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100. DAPI (blue).

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  IF Staining of C6 using CL594-66043

  Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-phalloidine (green).

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  IF Staining of C6 using CL594-66043

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-phalloidine (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: C6-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66043 bindet in IF LONP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LONP1 fusion protein Ag7306
• Vollständiger Name: lon peptidase 1, mitochondrial
• Berechnetes Molekulargewicht: 106 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC000235
• Gene symbol: LONP1
• Gene ID (NCBI): 9361
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).