LHCGR Polyklonaler Antikörper

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• KD/KO Validated

LHCGR Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 26424-1-AP

Synonyme

LSH-R, LHRHR, LHR, LH/CG-R, LGR2

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  WB analysis of mouse ovary using 26424-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 26424-1-AP (LHCGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 26424-1-AP (LHCGR antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse ovary using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 26424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse ovary using 26424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (26424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Eierstockgewebe, humanes Schilddrüsenkarzinomgewebe, Maus-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Eierstockgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

26424-1-AP bindet in WB, IHC, IF-P, ELISA LHCGR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LHCGR fusion protein Ag19259
• Vollständiger Name: LHCGR
• Berechnetes Molekulargewicht: 699 aa, 79 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC157028
• Gene symbol: LHCGR
• Gene ID (NCBI): 3973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Publikationen

Species	Application	Title
		Biol Reprod; Interferon-tau infusion into the ovine corpus luteum delays luteolysis; Authors - Aydin Guzeloglu
mouse	WB,IF	Biochem Biophys Res Commun; Effects of hCG on DA neuronal death of Parkinson's disease; Authors - Shi-Min Wang ; KD Validated
mouse	IF	Biol Res; Mouse testicular macrophages can independently produce testosterone and are regulated by Cebpb; Authors - Nengliang Duan