LGR5 Polyklonaler Antikörper

LGR5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 30007-1-AP

Synonyme

FEX, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67

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  WB analysis using 30007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30007-1-AP (LGR5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30007-1-AP (LGR5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BGC-823-Zellen, HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe, SGC-7901-Zellen, SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

30007-1-AP bindet in WB, IHC, IF, ELISA LGR5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGR5 fusion protein Ag31625
• Vollständiger Name: leucine-rich repeat-containing G protein-coupled receptor 5
• Berechnetes Molekulargewicht: 907 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC096324
• Gene symbol: LGR5
• Gene ID (NCBI): 8549
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins are the biological ligands of LGR5. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.LGR5 is crucial during embryogenesis as LGR null studies in mice incurred 100% neonatal mortality accompanied by several craniofacial distortions such as ankyloglossia and gastrointestinal dilation (PMID: 9642114, 16575208, 525477).

Publikationen

Species	Application	Title
mouse	IHC	Burns Trauma; Nocardia rubra cell-wall skeleton mitigates whole abdominal irradiation-induced intestinal injury via regulating macrophage function; Authors - Lingling Wu
mouse	IF	Acta Histochem; Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells; Authors - Yang Jiao
mouse	IF	Zool Res; Peptide Cy RL-QN15 accelerates hair regeneration in diabetic mice by binding to the Frizzled-7 receptor; Authors - Yu-Tong Wu
	IF	Int J Biol Macromol; Integrated bulk and single-cell RNA sequencing unveils the temporal and spatial dynamics of epidermal cell adhesion; Authors - Qingbo Zheng