LEO1 Polyklonaler Antikörper

LEO1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12281-1-AP

Synonyme

RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, RDL

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Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 12281-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12281-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12281-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12281-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12281-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12281-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12281-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 12281-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12281-1-AP (LEO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 12281-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12281-1-AP (LEO1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12281-1-AP

  Immunofluorescent analysis of Hela cells, using LEO1 antibody 12281-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, HL-60-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

12281-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LEO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LEO1 fusion protein Ag2932
• Vollständiger Name: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 666 aa, 75 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC018147
• Gene symbol: LEO1
• Gene ID (NCBI): 123169
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LEO1, also named as RNA polymerase-associated protein LEO1, is a 666 amino acid protein, which belongs to the LEO1 family. LEO1 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. The calculated molecular weight of LEO1 is 75 kDa, but the modified LEO1 protein is about 105 kDa (PMID: 15632063).

Publikationen

Species	Application	Title
human	WB	Nat Neurosci; Toxic expanded GGGGCC repeat transcription is mediated by the PAF1 complex in C9orf72-associated FTD.; Authors - Lindsey D Goodman
mouse	WB	Cell Death Dis; UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.; Authors - Gang Xiang
human	WB	Sci Adv; CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation; Authors - Min Qiu
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao