LCK Polyklonaler Antikörper

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• KD/KO Validated

LCK Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12477-1-AP

Synonyme

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 12477-1-AP

  WB result of LCK antibody (12477-1-AP, 1:2000) with si-control and si-LCK transfected Jurkat cells.

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  WB analysis of Jurkat using 12477-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12477-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12477-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12477-1-AP (LCK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12477-1-AP

  IP result of anti-LCK (IP:12477-1-AP, 4ug; Detection:12477-1-AP 1:500) with Jurkat cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

12477-1-AP bindet in WB, IP, ELISA LCK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LCK fusion protein Ag3176
• Vollständiger Name: lymphocyte-specific protein tyrosine kinase
• Berechnetes Molekulargewicht: 539 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC013200
• Gene symbol: LCK
• Gene ID (NCBI): 3932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	J Ovarian Res; Pretreatment with LCK inhibitors chemosensitizes cisplatin-resistant endometrioid ovarian tumors.; Authors - Katie K Crean-Tate
mouse	WB	Front Pharmacol; "Drying effect" of fructus aurantii components and the mechanism of action based on network pharmacology and in vitro pharmacodynamic validation; Authors - Jing Zhu
human	WB	J Ovarian Res; LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer; Authors - Goutam Dey
mouse	WB	Nat Commun; Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function; Authors - Yuman Wang