LCA5 Polyklonaler Antikörper

LCA5 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 19333-1-AP

Synonyme

C6orf152, LCA5, Leber congenital amaurosis 5, Lebercilin

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 19333-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19333-1-AP (LCA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

19333-1-AP bindet in WB, IF, CoIP, ELISA LCA5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LCA5 fusion protein Ag5140
• Vollständiger Name: Leber congenital amaurosis 5
• Berechnetes Molekulargewicht: 693 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC050327
• Gene symbol: LCA5
• Gene ID (NCBI): 167691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LCA5 (Leber congenital amaurosis 5), also named as C6orf152 or Lebercilin, is a 697 amino acids protein. LCA5 is widely expressed at the microtubules, centrosome, and primary cilia. Recent study showed that LCA5 was involved in the cause of congenital and early-onset retinal dystrophies (PMID: 24144451). The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.

Publikationen

Species	Application	Title
mouse		Mol Ther; Amelioration of Neurosensory Structure and Function in Animal and Cellular Models of a Congenital Blindness.; Authors - Ji Yun Song
mouse	IF	Hum Mol Genet; Disruption of the Retinitis Pigmentosa 28 gene Fam161a in mice affects photoreceptor ciliary structure and leads to progressive retinal degeneration.; Authors - Marcus Karlstetter
mouse	IF	Invest Ophthalmol Vis Sci; Treatment Potential for LCA5-Associated Leber Congenital Amaurosis.; Authors - Katherine E Uyhazi
pig	IF	J Proteomics; Tissue- and isoform-specific protein complex analysis with natively processed bait proteins.; Authors - Tina Beyer
human	WB,IF,CoIP	Sci Rep; The interaction between LC8 and LCA5 reveals a novel oligomerization function of LC8 in the ciliary-centrosome system; Authors - Tamás Szaniszló
human	IF	Nat Commun; iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy; Authors - Vincent Louvel