LBR Polyklonaler Antikörper

LBR Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 12398-1-AP

Synonyme

DHCR14B, Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta

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Galerie der Validierungsdaten

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  WB analysis using 12398-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12398-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 12398-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12398-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12398-1-AP

  IP result of anti-LBR (IP:12398-1-AP, 3ug; Detection:12398-1-AP 1:500) with A375 cells lysate 3200ug.

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  IF Staining of HeLa using 12398-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (12398-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of C6 using 12398-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using 12398-1-AP (LBR antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12398-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12398-1-AP (LBR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12398-1-AP

  Immunofluorescent analysis of HepG2 cells, using LBR antibody 12398-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 12398-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LBR (12398-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, C2C12-Zellen, HepG2-Zellen, humanes Hirngewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, C6-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12398-1-AP bindet in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA LBR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LBR fusion protein Ag3088
• Vollständiger Name: lamin B receptor
• Berechnetes Molekulargewicht: 615 aa, 71 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC020079
• Gene symbol: LBR
• Gene ID (NCBI): 3930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).

Publikationen

Species	Application	Title
mouse	IF	Mol Cell; Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy.; Authors - Lulu Jiang
		Nat Commun; PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.; Authors - Chen-Yu Wang
human	WB	PLoS Genet; RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.; Authors - Aurelio Reyes
human	IF	J Cell Sci; Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.; Authors - Janine Pfaff
human	WB,IP	J Cell Mol Med; Novel long non-coding RNA CYB561-5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non-small cell lung cancer.; Authors - Longfei Li
human	WB,IF	Mol Cell Biol; Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.; Authors - Hongli An