LATS1 Polyklonaler Antikörper

LATS1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 30174-1-AP

Synonyme

h warts, LATS1, WARTS, WARTS protein kinase, wts

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Galerie der Validierungsdaten

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  WB analysis using 30174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30174-1-AP (LATS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30174-1-AP (LATS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HepG2-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

30174-1-AP bindet in WB, ELISA LATS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LATS1 fusion protein Ag32879
• Vollständiger Name: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC002767
• Gene symbol: LATS1
• Gene ID (NCBI): 9113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Res Commun; IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression; Authors - Yan Xu