LAT Polyklonaler Antikörper

LAT Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11326-1-AP

Synonyme

pp36, p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11326-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11326-1-AP (LAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11326-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11326-1-AP (LAT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11326-1-AP

  IP result of anti-LAT (IP:11326-1-AP, 4ug; Detection:11326-1-AP 1:1000) with Jurkat cells lysate 3600ug.

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  IHC staining of human thymus using 11326-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11326-1-AP (LAT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 11326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LAT antibody (11326-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, mouse thymus
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Thymusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

11326-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LAT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAT fusion protein Ag1868
• Vollständiger Name: linker for activation of T cells
• Berechnetes Molekulargewicht: 262 aa, 28 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: BC011563
• Gene symbol: LAT
• Gene ID (NCBI): 27040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. LAT plays a central role in T-cell activation by nucleating signaling complexes that are critical for the propagation of T-cell signals from the plasma membrane to the cellular interior. In addition to being a positive regulator of T cell signaling, LAT also recruits several negative regulatory proteins, including kinases, phosphatases, and ubiquitin ligases, which ultimately leads to signal termination. LAT is subject to ubiquitylation, and ubiquitin-resistant mutants of LAT display enhanced signaling.

Publikationen

Species	Application	Title
human	WB	Front Pharmacol; HIF1α/VEGF Feedback Loop Contributes to 5-Fluorouracil Resistance.; Authors - Bin Shi
human	IHC	Sci Rep; A ceRNA-associated risk model predicts the poor prognosis for head and neck squamous cell carcinoma patients.; Authors - Yuzi Xu
human	IHC	Hepatol Int; Upregulation of CD147 protects hepatocellular carcinoma cell from apoptosis through glycolytic switch via HIF-1 and MCT-4 under hypoxia.; Authors - Xia Ke
human	WB	Eur J Haematol; Interferon gamma regulates a complex pro-survival signal network in chronic lymphocytic leukemia; Authors - Yixiang Chen
mouse		Sci China Life Sci; Phenylalanine diminishes M1 macrophage inflammation; Authors - Qingzhuo Zhang
mouse	WB	Nat Commun; Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function; Authors - Yuman Wang