LARP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LARP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

drosophila, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13708-1-AP

Synonyme

LARP, La-related protein 1, La related protein 1, KIAA0731

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Galerie der Validierungsdaten

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  WB analysis of HEK-293T using 13708-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13708-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13708-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13708-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13708-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13708-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13708-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13708-1-AP (LARP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13708-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LARP1 antibody (13708-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, Maushirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

13708-1-AP bindet in WB, IHC, IF/ICC, ELISA LARP1 und zeigt Reaktivität mit drosophila, human, Maus, Ratten

• Getestete Reaktivität: drosophila, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LARP1 fusion protein Ag4645
• Vollständiger Name: La ribonucleoprotein domain family, member 1
• Berechnetes Molekulargewicht: 1096 aa, 124 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC033856
• Gene symbol: LARP1
• Gene ID (NCBI): 23367
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.; Authors - Pedro Fuentes ; KO Validated
human	WB	Mol Cell Proteomics; A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications; Authors - Yuan-Qin Min
mouse	WB	Mol Autism; Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.; Authors - Annie Hien