LAMA4 (Isoform 3) Polyklonaler Antikörper

LAMA4 (Isoform 3) Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10465-1-AP

Synonyme

LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4

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Galerie der Validierungsdaten

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  WB analysis of human heart using 10465-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10465-1-AP (LAMA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 10465-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10465-1-AP (LAMA4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 10465-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10465-1-AP (LAMA4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 10465-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10465-1-AP (LAMA4 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 6 publications below

Produktinformation

10465-1-AP bindet in WB, IHC, ELISA LAMA4 (Isoform 3) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LAMA4 (Isoform 3) fusion protein Ag0736
• Vollständiger Name: laminin, alpha 4
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 11-13 kDa
• GenBank-Zugangsnummer: BC004241
• Gene symbol: Laminin alpha 4/LAMA4
• Gene ID (NCBI): 3910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363). Three alternatively spliced mRNAs give rise to three isoforms. This antibody was generated against the full length (1-120aa) of isoform 3.

Publikationen

Species	Application	Title
human	IHC	Arthritis Rheum; PMID: 21337318; Authors - Gutierrez-Roelens I I
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
mouse	IHC	Am J Physiol Heart Circ Physiol; Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.; Authors - Li Lin
human	IHC	Neuromuscul Disord; Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis.; Authors - Doppler K K
human	IHC	J Neurosci Res; Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone.; Authors - Xu Hongmin H
human	WB,IHC	Cancer Cell Int; MiR-539 inhibits proliferation and migration of triple-negative breast cancer cells by down-regulating LAMA4 expression.; Authors - Zhi-Xue Yang