L-VEGFA Polyklonaler Antikörper

L-VEGFA Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 22341-1-AP

Synonyme

L VEGFA, L-VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 22341-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 22341-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

22341-1-AP bindet in WB, IHC, ELISA L-VEGFA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: L-VEGFA fusion protein Ag17831
• Vollständiger Name: vascular endothelial growth factor A
• Berechnetes Molekulargewicht: 412 aa, 46 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC065522
• Gene symbol: VEGFA
• Gene ID (NCBI): 7422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1).

Publikationen

Species	Application	Title
human	WB	Mol Carcinog; PMID: 28833603; Authors - Hui-Hui Zhang
mouse	WB	Life Sci; Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy.; Authors - Jing Zou
		Tumour Biol; Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis.; Authors - Le Shi