KYNU Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KYNU Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 11796-1-AP

Synonyme

L-kynurenine hydrolase, L kynurenine hydrolase, Kynureninase, EC:3.7.1.3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11796-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11796-1-AP (KYNU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11796-1-AP (KYNU antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11796-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11796-1-AP (KYNU antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11796-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11796-1-AP (KYNU antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11796-1-AP

  Immunofluorescent analysis of HepG2 cells, using KYNU antibody 11796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 11796-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KYNU antibody (11796-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

11796-1-AP bindet in WB, IHC, IF/ICC, ELISA KYNU und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KYNU fusion protein Ag2365
• Vollständiger Name: kynureninase (L-kynurenine hydrolase)
• Berechnetes Molekulargewicht: 465aa,52 kDa; 307aa,35 kDa
• Beobachtetes Molekulargewicht: 45-52 kDa
• GenBank-Zugangsnummer: BC000879
• Gene symbol: KYNU
• Gene ID (NCBI): 8942
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755). This protein is a 95-kD kD homodimer predominantly located in the cytoplasm(PMID:9180257). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	IF	J Hazard Mater; Particulate matter-induced metabolic recoding of epigenetics in macrophages drives pathogenesis of chronic obstructive pulmonary disease; Authors - Myungkyung Noh
human	WB	Virol Sin; Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.; Authors - Yingying Song
rat	WB	Chem Res Toxicol; Tryptophan Pathway-Targeted Metabolomics Study on the Mechanism and Intervention of Cisplatin-Induced Acute Kidney Injury in Rats.; Authors - Bei Tan
human	WB	Clin Exp Dermatol; Downregulation of kynureninase restrains cutaneous squamous cell carcinoma proliferation and represses the PI3K/AKT pathway.; Authors - C Ci ; KD Validated
human	WB	Adv Sci (Weinh); Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth; Authors - Dong Liu ; KO Validated
human	WB,IHC	Oncogene; Glutathione peroxidase 2 knockdown suppresses gastric cancer progression and metastasis via regulation of kynurenine metabolism; Authors - Handong Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hala (Verified Customer) (03-29-2022)

it works good in BSA 5% dissolved in TBST

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hela cells

KYNU Antibody Western Blot validation (1/1000 dilution) in hela cells (Cat no:11796-1-AP)