Cytokeratin 20 Rekombinanter Antikörper

Cytokeratin 20 Rekombinant Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H10

Kat-Nr. : 82428-1-RR

Synonyme

CK 20, CK20, Cytokeratin 20, K20, keratin 20, KRT20, KRT21, Protein IT

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Galerie der Validierungsdaten

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  WB analysis using 82428-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 82428-1-RR

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 82428-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HT-29 using 82428-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 82428-1-RR

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, COLO 320-Zellen, Hausschwein-Kolongewebe, Maus-Kolongewebe, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, humanes Kolonkarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HT-29-Zellen
• Erfolgreiche Detektion in FC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:750-1:3000
• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82428-1-RR bindet in WB, IHC, IF, FC, ELISA Cytokeratin 20 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cytokeratin 20 fusion protein Ag11308
• Vollständiger Name: keratin 20
• Berechnetes Molekulargewicht: 424 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC031559
• Gene symbol: KRT20
• Gene ID (NCBI): 54474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.