Cytokeratin 18 Rekombinanter Antikörper

Cytokeratin 18 Rekombinant Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4F2

Kat-Nr. : 82817-5-RR

Synonyme

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie der Validierungsdaten

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  WB analysis using 82817-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82817-5-RR (KRT18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human appendicitis using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 82817-5-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82817-5-RR (Cytokeratin 18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HCT 116-Zellen, HepG2-Zellen, K-562-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, humanes Lebergewebe, humanes Mammakarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82817-5-RR bindet in WB, IHC, ELISA Cytokeratin 18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Vollständiger Name: keratin 18
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000180
• Gene symbol: KRT18
• Gene ID (NCBI): 3875
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 18, also known as CK18, is a type I intermediate filament protein which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. In the absence of CK8, the CK18 protein is degraded and keratin intermediate filaments are not formed.