Cytokeratin 13 Polyklonaler Antikörper

Cytokeratin 13 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 10164-2-AP

Synonyme

KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13

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Galerie der Validierungsdaten

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  WB analysis of A431 using 10164-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10164-2-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10164-2-AP

  IP result of anti-Cytokeratin 13 (IP:10164-2-AP, 4ug; Detection:10164-2-AP 1:1000) with A431 cells lysate 2400ug.

• 

  IHC staining of human cervical cancer using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human oesophagus using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human oesophagus using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HaCaT using 10164-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (10164-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10164-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 13 antibody (10164-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 10164-2-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 13 (10164-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushautgewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Ösophagusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HaCaT-Zellen, A431-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Produktinformation

10164-2-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Cytokeratin 13 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 13 fusion protein Ag0217
• Vollständiger Name: keratin 13
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002661
• Gene symbol: Cytokeratin 13
• Gene ID (NCBI): 3860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

Publikationen

Species	Application	Title
mouse	IF	Theranostics; Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p; Authors - Soo Hyun Kang
human	IF	Elife; Chloride channels regulate differentiation and barrier functions of the mammalian airway.; Authors - Mu He
human	WB	Cell Commun Signal; Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.; Authors - Henna Pehkonen
human	IF	Am J Respir Cell Mol Biol; Do airway epithelium air-liquid cultures represent the in vivo airway epithelium transcriptome?; Authors - Dvorak Anna A
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
human	IHC	Mol Vis; Expression analysis of human pterygium shows a predominance of conjunctival and limbal markers and genes associated with cell migration.; Authors - Jaworski C J CJ