Importin Beta Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Importin Beta Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 10077-1-AP

Synonyme

KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90

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Galerie der Validierungsdaten

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  WB analysis using 10077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10077-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 10077-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10077-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10077-1-AP (Importin beta antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 10077-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10077-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10077-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10077-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (10077-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, C6-Zellen, HeLa-Zellen, HT-1080.Zellen, Maushirngewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

10077-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA Importin Beta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Importin Beta fusion protein Ag0122
• Vollständiger Name: karyopherin (importin) beta 1
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 90-97 kDa
• GenBank-Zugangsnummer: BC003572
• Gene symbol: KPNB1
• Gene ID (NCBI): 3837
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

Publikationen

Species	Application	Title
human	WB,IP	Cell Res; Neddylation of PTEN regulates its nuclear import and promotes tumor development.; Authors - Ping Xie
human	WB	Oncogene; KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.; Authors - Zhi-Chuan Zhu ; KD Validated
human	WB,IP	J Transl Med; TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis; Authors - Xiang-Lin Hao
human	WB	Virol Sin; African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.; Authors - Yisha Zhuo
human	WB	PLoS Pathog; Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.; Authors - Weiyu Luo
human	WB	Biochem Biophys Res Commun; Nuclear import of Nkx2-2 is mediated by multiple pathways.; Authors - Wenbo Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works for WB (1:1000) and IF in permeabilized cells (1:100 ON). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC