Kallikrein 5 Polyklonaler Antikörper

Kallikrein 5 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10514-2-AP

Synonyme

Kallikrein 5, Kallikrein like protein 2, kallikrein related peptidase 5, KLK L2, KLK5, KLKL2, SCTE, Stratum corneum tryptic enzyme

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Galerie der Validierungsdaten

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  IHC staining of human skin using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10514-2-AP (Kallikrein 5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10514-2-AP (Kallikrein 5 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse skin using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10514-2-AP (Kallikrein 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10514-2-AP (Kallikrein 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10514-2-AP (Kallikrein 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10514-2-AP (Kallikrein 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Hautgewebe, Maushautgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

10514-2-AP bindet in WB, IF, IHC, ELISA Kallikrein 5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kallikrein 5 fusion protein Ag0799
• Vollständiger Name: kallikrein-related peptidase 5
• Berechnetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC008036
• Gene symbol: KLK5
• Gene ID (NCBI): 25818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLK5(Kallikrein-5) is also named as SCTE and belongs to the kallikrein subfamily. This protein is predicted to be a secreted serine protease, and the enzyme is found to have proteolytic activity. In serum and ascites fluid, the protein is present in two forms, one at a relatively lower molecular mass (around 50 kDa), and another one around 150-180 kDa and native KLK5 is highly glycosylated or that it may interact with the gel filtration column, leading to delayed retention(PMID:12873991). The activation of the enzyme has been shown to require cleavage of an arginine residue (Arg66-Ile67), suggesting that a trypsin-like serine protease may be involved in this process(PMID:15713679).

Publikationen

Species	Application	Title
human	IHC	Cancer Biomark; Salivary KLK5 and uPA are potential biomarkers for malignant transformation of OLK and OLP; Authors - Yingzhu Kang
human	WB,IF	J Dermatol Sci; Dichotomous effect of ultraviolet B on the expression of corneodesmosomal enzymes in human epidermal keratinocytes.; Authors - Nin Megumi M
human	WB,IHC	Cell Death Dis; Long noncoding RNA HEIH depletion depresses esophageal carcinoma cell progression by upregulating microRNA-185 and downregulating KLK5.; Authors - Bing Wang