KLK3/PSA Polyklonaler Antikörper

KLK3/PSA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 10679-1-AP

Synonyme

KLK3, KLK2A1, Kallikrein-3, Kallikrein 3, Gamma-seminoprotein

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Galerie der Validierungsdaten

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  WB analysis using 10679-1-AP

  DU 145(PSA-), PC-3(PSA-) and LNCaP (PSA+) cell lysates were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 10679-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10679-1-AP (KLK3/PSA antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of LNCaP using 10679-1-AP

  IP result of anti-KLK3/PSA (IP:10679-1-AP, 4ug; Detection:10679-1-AP 1:10000) with LNCaP cells lysate 1520 ug.

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  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10679-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10679-1-AP (KLK3/PSA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of LNCaP using 10679-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using KLK3/PSA antibody (10679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

10679-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA KLK3/PSA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLK3/PSA fusion protein Ag1060
• Vollständiger Name: kallikrein-related peptidase 3
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC005307
• Gene symbol: KLK3
• Gene ID (NCBI): 354
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms  produced by alternative splicing.

Publikationen

Species	Application	Title
human	IHC	Theranostics; ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.; Authors - Lijie Zhou
mouse	IHC	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	J Transl Med; CAPN2 promotes apalutamide resistance in metastatic hormone-sensitive prostate cancer by activating protective autophagy; Authors - Zihao Qi
human	WB	Elife; A feedback loop between the androgen receptor and 6-phosphogluoconate dehydrogenase (6PGD) drives prostate cancer growth; Authors - Joanna L Gillis
human	WB	Mol Cancer Ther; PMID: 28802253; Authors - Linh N K Tran
mouse	WB,IF	Stem Cell Res Ther; Expansion of mouse castration-resistant intermediate prostate stem cells in vitro; Authors - Yalan Xu