Kallikrein 11 Polyklonaler Antikörper

Kallikrein 11 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12698-1-AP

Synonyme

KLK11, KLK 11, Kallikrein-11 inactive chain 2, Kallikrein-11 inactive chain 1, Kallikrein-11

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 12698-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12698-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12698-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of PC-3 using 12698-1-AP

  Immunofluorescent analysis of PC-3 cells using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HeLa-Zellen, PC-3-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12698-1-AP bindet in WB, IHC, IF/ICC, ELISA Kallikrein 11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kallikrein 11 fusion protein Ag3427
• Vollständiger Name: kallikrein-related peptidase 11
• Berechnetes Molekulargewicht: 250 aa, 27 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC022068
• Gene symbol: KLK11
• Gene ID (NCBI): 11012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.