KLF13 Polyklonaler Antikörper

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• KD/KO Validated

KLF13 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 18352-1-AP

Synonyme

BTE binding protein 3, BTEB3, FKLF2, KLF13, Krueppel like factor 13, Kruppel like factor 13, NSLP1, RFLAT 1, RFLAT1, Transcription factor BTEB3, Transcription factor NSLP1

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Galerie der Validierungsdaten

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  WB analysis using 18352-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18352-1-AP (KLF13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 18352-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18352-1-AP (KLF13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18352-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18352-1-AP (KLF13 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

18352-1-AP bindet in WB, IHC, IF, ELISA KLF13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLF13 fusion protein Ag13200
• Vollständiger Name: Kruppel-like factor 13
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 37-45 kDa
• GenBank-Zugangsnummer: BC012741
• Gene symbol: KLF13
• Gene ID (NCBI): 51621
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kruppel-like factor 13(KLF13) is a member of transcription actors, which contains 3 zinc finger DNA-binding domains. Theses transcription factors bind to CG-rich sequences and related GT and CACCC boxes. KLF13 involved in activation of transcription of variety of genes in the cells of erythroid lineage, such as GATA1, glycophorin B. It also binds to the A and A/B RANTES promoter binding sites, the same as NFKB, and then transactivates the RANTES gene. The calculated molecular weight of KLF13 is 31 kDa, but the modified KLF13 protein is about 37-45 kDa.

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB,IF	J Hepatocell Carcinoma; Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.; Authors - Xingming Xie
mouse	WB,IF	J Biol Chem; Krüppel-Like Factor 13 is a major mediator of Glucocorticoid Receptor-Signaling in Cardiomyocytes and protects these Cells from DNA Damage and Death.; Authors - Diana Cruz-Topete
mouse	WB	Hum Cell; Downregulation of miR-96 suppresses the profibrogenic functions of cardiac fibroblasts induced by angiotensin II and attenuates atrial fibrosis by upregulating KLF13.; Authors - Lijie Su ; KD Validated
human	WB	Cell Biosci; KLF13 suppresses the proliferation and growth of colorectal cancer cells through transcriptionally inhibiting HMGCS1-mediated cholesterol biosynthesis.; Authors - Weilong Yao ; KD Validated
human	WB	Onco Targets Ther; Downregulation of KLF13 through DNMT1-mediated hypermethylation promotes glioma cell proliferation and invasion.; Authors - Rile Wu