KLF12 Polyklonaler Antikörper

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• KD/KO Validated

KLF12 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 13156-1-AP

Synonyme

AP 2rep, AP2REP, HSPC122, KLF12, Krueppel like factor 12, Kruppel like factor 12

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Galerie der Validierungsdaten

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  WB analysis using 13156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13156-1-AP (KLF12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13156-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13156-1-AP (KLF12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Nierengewebe, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

13156-1-AP bindet in WB, IHC, ChIP, ELISA KLF12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KLF12 fusion protein Ag3788
• Vollständiger Name: Kruppel-like factor 12
• Berechnetes Molekulargewicht: 402 aa, 44 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC019680
• Gene symbol: KLF12
• Gene ID (NCBI): 11278
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KLF12, also named as AP2REP or HSPC122, is a 402 amino acid protein, which contains three C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. KLF12 localizes in the nucleus and confers strong transcriptional repression to the AP-2-alpha gene. KLF12 is atranscriptional silencer to the AP2-alpha gene, mediating positive and negative regulation of the AP2-A expression trhough a set of overlapping cis-regulatory promoters elements.

Publikationen

Species	Application	Title
human	WB	Oncogene; Tumour-suppression function of KLF12 through regulation of anoikis.; Authors - N Godin-Heymann ; KD Validated
human	WB	Front Oncol; circ_NOTCH3 Functions as a Protooncogene Competing With miR-205-5p, Modulating KLF12 Expression and Promoting the Development and Progression of Basal-Like Breast Carcinoma.; Authors - Bing Guan
mouse	WB	J Ethnopharmacol; Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose.; Authors - Jing Zhou
human	WB	Onco Targets Ther; The role of miR-382-5p in glioma cell proliferation, migration and invasion.; Authors - Jinjin Wang
human	WB,IHC	Biochem Biophys Res Commun; Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization.; Authors - Shen Xiaoyue X
mouse	WB	Genes Dis; All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members.; Authors - Jiejie Cui