Sam68 Polyklonaler Antikörper

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• KD/KO Validated

Sam68 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 10222-1-AP

Synonyme

KHDRBS1, Src-associated in mitosis 68 kDa protein, p21 Ras GTPase-activating protein-associated p62, KHDRBS 1, KH domain-containing, RNA-binding, signal transduction-associated protein 1

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Galerie der Validierungsdaten

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  WB analysis using 10222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10222-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10222-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10222-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10222-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10222-1-AP

  IP result of anti-Sam68 (IP:10222-1-AP, 4ug; Detection:10222-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 10222-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10222-1-AP (KHDRBS1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse brain using 10222-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10222-1-AP (Sam68 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HT-29-Zellen, Mausembryogewebe, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

10222-1-AP bindet in WB, IHC, IF-P, IP, CoIP, RIP, ELISA Sam68 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sam68 fusion protein Ag0235
• Vollständiger Name: KH domain containing, RNA binding, signal transduction associated 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC000717
• Gene symbol: Sam68
• Gene ID (NCBI): 10657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and INS receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. KHDRBS1 has three isoforms with calculated MW of 44-48kd. For isoform with a calculated MW of 48.2 kD, it migrated as a 68kD protein on SDS-PAGE gels[PMID: 10564820]. This ia a rabbit polyclonal antibody raised against residues near the N terminus of human KHDRBS1.

Publikationen

Species	Application	Title
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
mouse	IHC	Brain; Regulatory T cells delay disease progression in Alzheimer-like pathology.; Authors - Cira Dansokho
human	IHC	Theranostics; SAM68 promotes tumorigenesis in lung adenocarcinoma by regulating metabolic conversion via PKM alternative splicing.; Authors - Song Zhu
human	WB,CoIP,RIP	Mol Ther Nucleic Acids; LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation; Authors - Jianbo Feng
human	WB	FASEB J; CircMTA1 promotes glioblastoma angiogenesis by encoding MTA1-134aa; Authors - Ben Niu
mouse	WB	Reprod Fertil Dev; Cold-induced RNA-binding protein (CIRBP) regulates the expression of Src-associated during mitosis of 68 kDa (Sam68) and extracellular signal-regulated kinases (ERK) during heat stress-induced testicular injury.; Authors - Chengcheng Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Julia (Verified Customer) (08-07-2024)

Used for 30ug of mESC. Many bands are visible with the thickest one at the correct size. Increasing the dilution should help to obtain cleaner picture.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESC

Sam68 Antibody Western Blot validation (1:3000 dilution) in mESC (Cat no:10222-1-AP)