KGA-Specific Monoklonaler Antikörper

KGA-Specific Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B11A11

Kat-Nr. : 66265-2-Ig

Synonyme

GLS, GLS1, glutaminase, KGA, KGA-Specific, L glutamine amidohydrolase

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 66265-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66265-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66265-2-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66265-2-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66265-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66265-2-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66265-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-2-Ig (GLS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66265-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-2-Ig (GLS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 66265-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KGA-Specific antibody (66265-2-Ig, Clone: 1B11A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66265-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KGA-Specific antibody (66265-2-Ig, Clone: 1B11A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, COLO 320-Zellen, HEK-293-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66265-2-Ig bindet in WB, IHC, IF, ELISA KGA-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KGA-Specific fusion protein Ag24227
• Vollständiger Name: glutaminase
• Berechnetes Molekulargewicht: 669 aa, 73 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC038507
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.