KDM4A Polyklonaler Antikörper

KDM4A Polyklonal Antikörper für FC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29977-1-AP

Synonyme

JHDM3A, JMJD2, JMJD2A, KDM4A, KIAA0677, lysine (K) demethylase 4A, Lysine specific demethylase 4A

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Galerie der Validierungsdaten

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  WB analysis using 29977-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 29977-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 29977-1-AP

  IP result of anti-KDM4A(IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with MCF-7 cells lysate 2120 ug.

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  IP experiment of A549 using 29977-1-AP

  IP result of anti-KDM4A(IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with A549 cells lysate 800 ug.

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  FC experiment of A549 using 29977-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KDM4A (29977-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Mauslungengewebe, MCF-7-Zellen, MDA-MB-231-Zellen, NCL-H1299-Zellen
• Erfolgreiche IP: MCF-7-Zellen, A549-Zellen
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29977-1-AP bindet in WB, IP, FC, ELISA KDM4A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KDM4A fusion protein Ag31992
• Vollständiger Name: lysine (K)-specific demethylase 4A
• Berechnetes Molekulargewicht: 1064 aa, 121 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: BC002558
• Gene symbol: KDM4A
• Gene ID (NCBI): 9682
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.