JNK Polyklonaler Antikörper
JNK Polyklonal Antikörper für IF, IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (3)
Anwendung
WB, IP, IHC, IF, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 51151-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | 4T1-Zellen, A431-Zellen, C6-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Neuro-2a-Zellen, NIH/3T3-Zellen |
Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | SH-SY5Y-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 115 publications below |
IHC | See 2 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Produktinformation
51151-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA JNK und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | mitogen-activated protein kinase 8 |
Berechnetes Molekulargewicht | 48 kDa |
Beobachtetes Molekulargewicht | 44-48 kDa, 50-55 kDa |
GenBank-Zugangsnummer | NM_138982 |
Gene symbol | MAPK8 |
Gene ID (NCBI) | 5599 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Function
JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.
Tissue specificity
JNK1 is ubiquitously expressed.
Involvement in disease
JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.
Isoforms
There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.
Post-translational modifications
JNK1 is phosphorylated by MAPK kinases (MAPKK).
Cellular localization
JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for JNK antibody 51151-1-AP | Protokoll herunterladen |
IHC protocol for JNK antibody 51151-1-AP | Protokoll herunterladen |
IF protocol for JNK antibody 51151-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Crohns Colitis Mincle/Syk signaling promotes intestinal mucosal inflammation through induction of macrophage pyroptosis in Crohn's disease. | ||
Br J Pharmacol Amentoflavone attenuates Listeria monocytogenes pathogenicity through an LLO-dependent mechanism. | ||
J Transl Med Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways | ||
Biochim Biophys Acta Mol Basis Dis Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages | ||
Aging (Albany NY) Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice. |