JNK Polyklonaler Antikörper

JNK Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51151-1-AP

Synonyme

c Jun N terminal kinase 1, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1



Geprüfte Anwendungen

Erfolgreiche Detektion in WB4T1-Zellen, A431-Zellen, C6-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Neuro-2a-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

51151-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA JNK und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name mitogen-activated protein kinase 8
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 44-48 kDa, 50-55 kDa
GenBank-ZugangsnummerNM_138982
Gene symbol MAPK8
Gene ID (NCBI) 5599
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease
  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Protokolle

Produktspezifische Protokolle
WB protocol for JNK antibody 51151-1-APProtokoll herunterladen
IHC protocol for JNK antibody 51151-1-APProtokoll herunterladen
IF protocol for JNK antibody 51151-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Res

Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis.

Authors - Wu Jianfeng J
mouseWB

J Crohns Colitis

Mincle/Syk signaling promotes intestinal mucosal inflammation through induction of macrophage pyroptosis in Crohn's disease.

Authors - Wenbin Gong
mouseWB

Br J Pharmacol

Amentoflavone attenuates Listeria monocytogenes pathogenicity through an LLO-dependent mechanism.

Authors - Wang Tingting
mouseWB

J Transl Med

Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways

Authors - Xin-Sen Chen
humanWB

Biochim Biophys Acta Mol Basis Dis

Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages

Authors - Ling Zhou
humanWB

Aging (Albany NY)

Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice.

Authors - Dongdong Huang