IkB Alpha Monoklonaler Antikörper

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• KD/KO Validated

IkB Alpha Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(41)

CloneNo.

2G6H7

Kat-Nr. : 66418-1-Ig

Synonyme

IKBA, IκB α, NFKBIA, IkB-alpha, I-kappa-B-alpha

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  WB analysis using 66418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66418-1-Ig

  WB result of IkB Alpha antibody (66418-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Alpha transfected HEK-293 cells.

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  WB analysis of C6 using 66418-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66418-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66418-1-Ig (IkB alpha antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66418-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66418-1-Ig (IkB alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Raji-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 39 publications below
• IF: See 2 publications below

Produktinformation

66418-1-Ig bindet in WB, IHC, IF, ELISA IkB Alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IkB Alpha fusion protein Ag22040
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004983
• Gene symbol: IkB Alpha
• Gene ID (NCBI): 4792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publikationen

Species	Application	Title
rat	WB	Acta Biomater; An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury.; Authors - Xu Liao
mouse	WB	Mol Ther Nucleic Acids; TAZ ameliorates the microglia-mediated inflammatory response via the Nrf2-ROS-NF-κB pathway.; Authors - Ji-Cheng Huang
human	WB	Bioengineered; Calcium-activated nucleotides 1 (CANT1)-driven nuclear factor-k-gene binding (NF-ĸB) signaling pathway facilitates the lung cancer progression; Authors - Fangfang Gao
human,mouse	WB	Int Immunopharmacol; OSU-T315 overcomes immunosuppression in triple-negative breast cancer by targeting the ILK/NF-κB signaling pathway to enhance immunotherapeutic efficacy; Authors - Yi Wang
mouse	WB	J Ethnopharmacol; Buyang Huanwu Decoction suppresses cardiac inflammation and fibrosis in mice after myocardial infarction through inhibition of the TLR4 signalling pathway; Authors - Guoyong Zhang
rat	WB	Foods; Gastroprotective Effects of the Aqueous Extract of Finger Citron Pickled Products against Ethanol-Induced Gastric Damage: In Vitro and In Vivo Studies; Authors - Xiaoai Chen