IVD Polyklonaler Antikörper

IVD Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 10822-1-AP

Synonyme

Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2

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Galerie der Validierungsdaten

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  WB analysis using 10822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10822-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10822-1-AP

  IP result of anti-IVD (IP:10822-1-AP, 4ug; Detection:10822-1-AP 1:300) with MCF-7 cells lysate 800ug.

• 

  IHC staining of human liver cancer using 10822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10822-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10822-1-AP (IVD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HepG2-Zellen, humanes Hirngewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

10822-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA IVD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: arabidopsis, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IVD fusion protein Ag1252
• Vollständiger Name: isovaleryl Coenzyme A dehydrogenase
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 41-46 kDa
• GenBank-Zugangsnummer: BC017202
• Gene symbol: IVD
• Gene ID (NCBI): 3712
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
Arabidopsis	WB	Plant J; PMID: 19929878; Authors - Gu Liping L
mouse	WB	Cell Mol Life Sci; Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.; Authors - Min-Zhi Peng
mouse	WB	Mol Endocrinol; Research resource: A dual proteomic approach identifies regulated islet proteins during beta cell mass expansion in vivo.; Authors - Signe Horn
mouse	WB	J Neurochem; Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure.; Authors - Ryu Myung-Jeom MJ
	WB	Plant Cell Rep; The role of the electron-transfer flavoprotein: ubiquinone oxidoreductase following carbohydrate starvation in Arabidopsis cell cultures.; Authors - Danielle S Brito
rat	WB	Proteomics Clin Appl; Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration.; Authors - Kelly L Stauch

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lana (Verified Customer) (06-21-2019)

Lines on WB:1 - BioRad Precision Plus Protein standards2 and 3 – biological replicates, lysates of mitochondrial fractions of ST-Hdh-Q7/Q7 mouse striatal cell line.SDS-PAGE:15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ST-Hdh mouse striatal cell line

IVD Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:10822-1-AP)