ITM2C Monoklonaler Antikörper

ITM2C Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Hund

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2E8G11

Kat-Nr. : CL594-60095

Synonyme

BRI3, BRICD2C, Cerebral protein 14, E25, E25C, integral membrane protein 2C, ITM2C, ITM3, PSEC0047, Transmembrane protein BRI3

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Galerie der Validierungsdaten

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  IF Staining of MDCK using CL594-60095

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using the CoraLite®594-conjugated version of this antibody, CL594-60095 (ITM2C antibody), at dilution of 1:100.

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  IF Staining of HepG2 using CL594-60095

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60095 (ITM2C antibody) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: MDCK-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60095 bindet in IF ITM2C und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ITM2C fusion protein Ag4409
• Vollständiger Name: integral membrane protein 2C
• Berechnetes Molekulargewicht: 230aa,26 kDa; 267aa,30 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC025742
• Gene symbol: ITM2C
• Gene ID (NCBI): 81618
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Integral membrane protein 2C (ITM2C) is a type II integral transmembrane protein belonging to a family composed of at least two other members, ITM2A and ITM2B. ITM2C, often called transmembrane protein BRI3, is highly expressed in brain tissue. Yeast two-hybrid screen system revealed that BRI3 could interact with beta-secretase beta-amyloid protein converting enzyme (BACE)1 and the microtubule-destabilizing protein SCG10 (STMN2). It may play a role in TNF-induced cell death and neuronal differentiation. BRI3 was found to inhibit the various processing of amyloid precursor protein (APP) by blocking the access of alpha- and beta-secretases to APP, competitive inhibition of APP processing by BRI3 may provide a new approach to Alzheimer disease (AD) therapy and prevention.