IRS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IRS1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IP, IHC, IF, FC, Dot blot, ELISA

Konjugation

Unkonjugiert

Publikationen

52

Kat-Nr. : 17509-1-AP

Synonyme

HIRS 1, IRS 1, IRS1, IRS-1

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Galerie der Validierungsdaten

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  WB analysis of A549 using 17509-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17509-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17509-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 17509-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 17509-1-AP

  IP Result of anti-IRS1 (IP:17509-1-AP, 5ug; Detection:17509-1-AP 1:1000) with A549 cells lysate 3500ug.

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  IHC staining of human breast cancer using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 17509-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A549 using 17509-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of MCF-7 using 17509-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human IRS1 (17509-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A431-Zellen, HEK-293-Zellen, PC-3-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

17509-1-AP bindet in WB, IP, IHC, IF, FC, Dot blot, ELISA IRS1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Riesenscheibenbrasse (Megalobrama amblycephala)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRS1 fusion protein Ag11714
• Vollständiger Name: insulin receptor substrate 1
• Berechnetes Molekulargewicht: 1242 aa, 132 kDa
• Beobachtetes Molekulargewicht: 160-185 kDa
• GenBank-Zugangsnummer: BC053895
• Gene symbol: IRS1
• Gene ID (NCBI): 3667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.

Publikationen

Species	Application	Title
human	WB	Mol Cell; KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis; Authors - Hiroshi Senoo
mouse	WB	Autophagy; BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.; Authors - Simone C da Silva Rosa
mouse	Dot blot	Environ Health Perspect; Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.; Authors - Jacob Raber
mouse	WB	JHEP Rep; Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis; Authors - Fuji Yang
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
mouse	WB	Elife; EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence.; Authors - Jeremie Gautheron