Inhibin Beta A Polyklonaler Antikörper

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• KD/KO Validated

Inhibin Beta A Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 10651-1-AP

Synonyme

Activin beta A chain, EDF, FRP, INHBA, Inhibin Beta A, Inhibin beta A chain, Inhibin β-A, inhibin, beta A

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Galerie der Validierungsdaten

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  WB analysis using 10651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10651-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10651-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10651-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10651-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 10651-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10651-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10651-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 10651-1-AP

  Immunofluorescent analysis of A549 cells using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, BGC-823-Zellen, HepG2-Zellen, MCF-7-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 11 publications below
• IF: See 3 publications below

Produktinformation

10651-1-AP bindet in WB, IF, IHC, ELISA Inhibin Beta A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Inhibin Beta A fusion protein Ag1001
• Vollständiger Name: inhibin, beta A
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC007858
• Gene symbol: Inhibin beta A
• Gene ID (NCBI): 3624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Inhibin is a 32-kD dimeric glycoprotein consisting of an alpha subunit and one of two beta subunits (beta A or beta B) leading to inhibin A or B, respectively, which was isolated and cloned on the basis of its ability to inhibit FSH release from the pituitary. Inhibin beta A forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin beta A can also bind the beta B and A isoforms to form activin AB and inhibin, respectively.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen
		Development; Combinatorial actions of Tgfβ and Activin ligands promote oligodendrocyte development and CNS myelination.; Authors - Dipankar J Dutta
mouse	WB	PLoS One; Action mechanism of inhibin α-subunit on the development of Sertoli cells and first wave of spermatogenesis in mice.; Authors - Cai Kailai K
human	IHC,IF	Transl Oncol; Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer; Authors - Nanxin Zheng
		Hepatol Commun; Activin A is a prominent autocrine regulator of hepatocyte growth arrest.; Authors - Srividyameena Haridoss
		BMC Cancer; Esophageal squamous cell carcinoma invasion is inhibited by Activin A in ACVRIB-positive cells.; Authors - Holli A Loomans

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